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pan cytokeratin cocktail mixture  (Bio-Rad)


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    Bio-Rad pan cytokeratin cocktail mixture
    Pan Cytokeratin Cocktail Mixture, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+pan+cd45+antibody/pmc11810791-224-12-28?v=Bio-Rad
    Average 93 stars, based on 140 article reviews
    pan cytokeratin cocktail mixture - by Bioz Stars, 2026-07
    93/100 stars

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    FIGURE 1 | Experimental design and correlation of viral loads with engraftment levels. (A) Illustrates the experimental design for the generation of hCD34+ humanized mice, HIV infection, groups implemented and the tissues harvested for the study. The time point for the tissue harvest reflects chronic infection. Representative flow cytometric dot plot of mouse blood-stained for human CD45-PerCP and mouse CD45-FITC used to identify successfully engrafted animals. The 2 × 2 design for female and male mice is indicated. (B) Correlation analyses between measured variables %hCD45+ cells (at time of engraftment) and viral loads (at study endpoint) for each mouse in the four HIV-infected groups. The Spearman coefficients (rs) indicated, while positive, did not reach significance (P < 0.05) in any of the groups. F-HIV-Opn+ n = 5, F-HIV-Opn– n = 9, M-HIV-Opn+ n = 8 and M-HIV-Opn– n = 5.

    Journal: Frontiers in Virology

    Article Title: Complex Interactions Between Human Immunodeficiency Virus Type-1, Sex, and Osteopontin Influence Viral Replication and Leukocyte Proportions in Tissues

    doi: 10.3389/fviro.2021.690360

    Figure Lengend Snippet: FIGURE 1 | Experimental design and correlation of viral loads with engraftment levels. (A) Illustrates the experimental design for the generation of hCD34+ humanized mice, HIV infection, groups implemented and the tissues harvested for the study. The time point for the tissue harvest reflects chronic infection. Representative flow cytometric dot plot of mouse blood-stained for human CD45-PerCP and mouse CD45-FITC used to identify successfully engrafted animals. The 2 × 2 design for female and male mice is indicated. (B) Correlation analyses between measured variables %hCD45+ cells (at time of engraftment) and viral loads (at study endpoint) for each mouse in the four HIV-infected groups. The Spearman coefficients (rs) indicated, while positive, did not reach significance (P < 0.05) in any of the groups. F-HIV-Opn+ n = 5, F-HIV-Opn– n = 9, M-HIV-Opn+ n = 8 and M-HIV-Opn– n = 5.

    Article Snippet: Tissues were evaluated at the study endpoint to validate and quantify human leukocytes by seven-color flow cytometry with a cocktail containing the following antibodies: human pan-CD45-Viogreen or PerCP (Miltenyi Biotec), 130-110-638, ThermoFisher (MHCD4531), CD3-PE (ThermoFisher, MHCD0304), CD4-Vioblue (Miltenyi Biotec, 130-113-219), CD8-APC-Vio770 (Miltenyi Biotec, 130- 113-155), CD14-APC (BioLegend, 301808), CD19-FITC or PerCP (eBiosciences, 11-0199-42; BioLegend, 302228) and mouse CD45-FITC (eBiosciences, 11-0451-82).

    Techniques: Infection, Staining